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National
United States Regulations
40 CFR PART 136—GUIDELINES ESTABLISHING TEST PROCEDURES FOR THE ANALYSIS OF POLLUTANTS



Title 40: Protection of Environment


PART 136—GUIDELINES ESTABLISHING TEST PROCEDURES FOR THE ANALYSIS OF POLLUTANTS



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Authority: Secs. 301, 304(h), 307 and 501(a), Pub. L. 95–217, 91 Stat. 1566, et seq. (33 U.S.C. 1251, et seq.) (the Federal Water Pollution Control Act Amendments of 1972 as amended by the Clean Water Act of 1977).

§ 136.1 Applicability.
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The procedures prescribed herein shall, except as noted in §136.5, be used to perform the measurements indicated whenever the waste constituent specified is required to be measured for:

(a) An application submitted to the Administrator, or to a State having an approved NPDES program for a permit under section 402 of the Clean Water Act of 1977, as amended (CWA), and/or to reports required to be submitted under NPDES permits or other requests for quantitative or qualitative effluent data under parts 122 to 125 of title 40, and,

(b) Reports required to be submitted by discharges under the NPDES established by parts 124 and 125 of this chapter, and,

(c) Certifications issued by States pursuant to section 401 of the CWA, as amended.

[38 FR 28758, Oct. 16, 1973, as amended at 49 FR 43250, Oct. 26, 1984]

§ 136.2 Definitions.
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As used in this part, the term:

(a) Act means the Clean Water Act of 1977, Pub. L. 95–217, 91 Stat. 1566, et seq. (33 U.S.C. 1251 et seq.) (The Federal Water Pollution Control Act Amendments of 1972 as amended by the Clean Water Act of 1977).

(b) Administrator means the Administrator of the U.S. Environmental Protection Agency.

(c) Regional Administrator means one of the EPA Regional Administrators.

(d) Director means the Director of the State Agency authorized to carry out an approved National Pollutant Discharge Elimination System Program under section 402 of the Act.

(e) National Pollutant Discharge Elimination System (NPDES) means the national system for the issuance of permits under section 402 of the Act and includes any State or interstate program which has been approved by the Administrator, in whole or in part, pursuant to section 402 of the Act.

(f) Detection limit means the minimum concentration of an analyte (substance) that can be measured and reported with a 99% confidence that the analyte concentration is greater than zero as determined by the procedure set forth at appendix B of this part.

[38 FR 28758, Oct. 16, 1973, as amended at 49 FR 43250, Oct. 26, 1984]

§ 136.3 Identification of test procedures.
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(a) Parameters or pollutants, for which methods are approved, are listed together with test procedure descriptions and references in Tables IA, IB, IC, ID, IE, and IF. The full text of the referenced test procedures are incorporated by reference into Tables IA, IB, IC, ID, IE, and IF. The incorporation by reference of these documents, as specified in paragraph (b) of this section, was approved by the Director of the Federal Register in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. Copies of the documents may be obtained from the sources listed in paragraph (b) of this section. Information regarding obtaining these documents can be obtained from the EPA Office of Water Statistics and Analytical Support Branch at 202–566–1000. Documents may be inspected at EPA's Water Docket, EPA West, 1301 Constitution Avenue, NW., Room B135, Washington, DC (Telephone: 202-566–2426); or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202–741–6030, or go to: http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. These test procedures are incorporated as they exist on the day of approval and a notice of anys change in these test procedures will be published in the Federal Register. The discharge parameter values for which reports are required must be determined by one of the standard analytical test procedures incorporated by reference and described in Tables IA, IB, IC, IE, and IF, or by any alternate test procedure which has been approved by the Administrator under the provisions of paragraph (d) of this section and §§136.4 and 136.5. Under certain circumstances (paragraph (b) or (c) of this section or 40 CFR 401.13) other test procedures may be more advantageous when such other test procedures have been previously approved by the Regional Administrator of the Region in which the discharge will occur, and providing the Director of the State in which such discharge will occur does not object to the use of such alternate test procedure.


Table IA_List of Approved Biological Methods
--------------------------------------------------------------------------------------------------------------------------------------------------------
Standard methods 18th,
Parameter and units Method \1\ EPA 19th, 20th Ed. ASTM AOAC USGS Other
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Bacteria:
1. Coliform (fecal), number Most Probable Number p. 132 9221C E \4\
per 100 mL. (MPN), 5 tube 3 \3\
dilution, or
Membrane filter (MF) p. 124 9222D \4\ ........... ........... B-0050-85
\2\, single step. \3\ \5\
2. Coliform (fecal) in MPN, 5 tube, 3 p. 132 9221C E \4\
presence of chlorine, number dilution, or \3\
per 100 mL.
MF, single step \6\. p. 124 9222D \4\
\3\
3. Coliform (total), number MPN, 5 tube, 3 p. 114 9221B \4\
per 100 mL. dilution, or \3\
MF \2\, single step p. 108 9222B \4\ ........... ........... B-0025-85
or two step. \3\ \5\
4. Coliform (total), in MPN, 5 tube, 3 p. 114 9221B \4\
presence of chlorine, number dilution, or \3\
per 100 mL.
MF \2\ with p. 111 9222(B+B.5c) \4\
enrichment. \3\
5. E. coli, number per 100 mL MPN \7,9,15\, ......... 9221B.1/9221F \4,12,14\
\28\. multiple tube,.
multiple tube/ ......... 9223B \4,13\ ........... 991.15 \11\ ........... Colilert ®
multiple well, \13,17\
Colilert-18 ®
\13,16,17\
MF \2,6,7,8,9\ two ......... 9222B/9222G \4,19\
step, or
1103.1 9213D \4\ D5392-93
\20\ \10\
single step......... 1603 \21\
1604 \22\
......... ....................... ........... ........... ........... mColiBue 24 \18\
6. Fecal streptococci, number MPN, 5 tube, 3 p. 139 9230B \4\, 9230C \4\
per 100 mL. dilution, \3\
MF \2\, or.......... p. 136 ....................... ........... B-0055-85
\3\ \5\
Plate count......... p. 143
\4\
7. Enterococci, number per MPN \7,9\ multiple ......... 9230B \4\
100 mL. tube.
multiple tube/ ......... ....................... D6503-99 ........... ........... Enterolert
multiple well. \10\ ®\13,23\
MF \2,6,7,8,9\ two 1106.1 9230C \4\ D5259-92
step. \24\ \10\
single step, or..... 1600 \25\
Plate count......... p. 143
\3\
Protozoa:
8. Cryptosporidium \28\...... Filtration/IMS/FA... 1622 \26\
1623 \27\
9. Giardia \28\.............. Filtration/IMS/FA... 1623 \27\
Aquatic Toxicity:
10. Toxicity, acute, fresh Ceriodaphnia dubia 2002.0
water organisms, LC50, acute. \29\
percent effluent.
Daphnia puplex and 2021.0
Daphnia magna acute. \29\
Fathead Minnow, 2000.0
Pimephales \29\
promelas, and
Bannerfin shiner,
Cyprinella leedsi,
acute.
Rainbow Trout, 2019.0
Oncorhynchus \29\
mykiss, and brook
trout, Salvelinus
fontinalis, acute.
11. Toxicity, acute, Mysid, Mysidopsis 2007.0
estuarine and marine bahia, acute. \29\
organisms of the Atlantic
Ocean and Gulf of Mexico,
LC50, percent effluent.
Sheepshead Minnow, 2004.0
Cyprinodon \29\
variegatus, acute.
Silverside, Menidia 2006.0
beryllina, Menidia \29\
menidia, and
Menidia peninsulae,
acute.
12. Toxicity, chronic, fresh Fathead minnow, 1000.0
water organisms, NOEC or Pimephales \30\
IC25, percent effluent. promelas, larval
survival and growth.
Fathead minnow, 1001.0
Pimephales \30\
promelas, embryo-
larval survival and
teratogenicity.
Daphnia, 1002.0
Ceriodaphnia dubia, \30\
survival and
reproduction.
Green alga, 1003.0
Selenastrum \30\
capricornutum,
growth.
13. Toxicity, chronic, Sheepshead minnow, 1004.0
estuarine and marine Cyprinodon \31\
organisms of the Atlantic variegatus, larval
Ocean and Gulf of Mexico, survival and growth.
NOEC or IC25, percent
effluent.
Sheepshead minnow, 1005.0
Cyprinodon \31\
variegatus, embryo-
larval survival and
teratogenicity.
Inland silverside, 1006.0
Menidia beryllina, \31\
larval survival and
growth.
Mysid, Mysidopsis 1007.0
bahia, survival, \31\
growth, and
fecundity.
Sea urchin, Arbacia 1008.0
punctulata, \31\
fertilization.
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Notes to Table IA:
\1\ The method must be specified when results are reported.
\2\ A 0.45 µm membrane filter (MF) or other pore size certified by the manufacturer to fully retain organisms to be cultivated and to be free of
extractables which could interfere with their growth.
\3\ USEPA. 1978. Microbiological Methods for Monitoring the Environment, Water, and Wastes. Environmental Monitoring and Support Laboratory, U.S.
Environmental Protection Agency, Cincinnati, Ohio. EPA/600/8-78/017.
\4\ APHA. 1998, 1995, 1992. Standard Methods for the Examination of Water and Wastewater. American Public Health Association. 20th, 19th, and 18th
Editions. Amer. Publ. Hlth. Assoc., Washington, D.C.
\5\ USGS. 1989. U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and
Analysis of Aquatic Biological and Microbiological Samples, U.S. Geological Survey, U.S. Department of Interior, Reston, Virginia.
\6\ Because the MF technique usually yields low and variable recovery from chlorinated wastewaters, the Most Probable Number method will be required to
resolve any controversies.
\7\ Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes
to account for the quality, character, consistency, and anticipated organism density of the water sample.
\8\ When the MF method has not been used previously to test ambient waters with high turbidity, large number of noncoliform bacteria, or samples that
may contain organisms stressed by chlorine, a parallel test should be conducted with a multiple-tube technique to demonstrate applicability and
comparability of results.
\9\ To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the
year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA
alternate test procedure (ATP) guidelines.
\10\ ASTM. 2000, 1999, 1996. Annual Book of ASTM Standards_Water and Environmental Technology. Section 11.02. American Society for Testing and
Materials. 100 Barr Harbor Drive, West Conshohocken, PA 19428.
\11\ AOAC. 1995. Official Methods of Analysis of AOAC International, 16th Edition, Volume I, Chapter 17. Association of Official Analytical Chemists
International. 481 North Frederick Avenue, Suite 500, Gaithersburg, Maryland 20877-2417.
\12\ The multiple-tube fermentation test is used in 9221B.1. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25 parallel
tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-positive rate
and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase on 10 percent
of all total coliform-positive tubes on a seasonal basis.
\13\ These tests are collectively known as defined enzyme substrate tests, where, for example, a substrate is used to detect the enzyme ß-
glucuronidase produced by E. coli.
\14\ After prior enrichment in a presumptive medium for total coliform using 9221B.1, all presumptive tubes or bottles showing any amount of gas, growth
or acidity within 48 h ±3 h of incubation shall be submitted to 9221F. Commercially available EC-MUG media or EC media supplemented in the
laboratory with 50 µg/mL of MUG may be used.
\15\ Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube procedures, employ an appropriate tube and
dilution configuration of the sample as needed and report the Most Probable Number (MPN). Samples tested with Colilert ® may be enumerated with
the multiple-well procedures, Quanti-Tray ® or Quanti-Tray ® 2000, and the MPN calculated from the table provided by the manufacturer.
\16\ Colilert-18 ® is an optimized formulation of the Colilert ® for the determination of total coliforms and E. coli that provides results
within 18 h of incubation at 35 °C rather than the 24 h required for the Colilert ® test and is recommended for marine water samples.
\17\ Descriptions of the Colilert ®, Colilert-18 ®, Quanti-Tray ®, and Quanti-Tray ®/2000 may be obtained from IDEXX Laboratories, Inc.,
One IDEXX Drive, Westbrook, Maine 04092.
\18\ A description of the mColiBlue24'' test, Total Coliforms and E. coli, is available from Hach Company, 100 Dayton Ave., Ames, IA 50010.
\19\ Subject total coliform positive samples determined by 9222B or other membrane filter procedure to 9222G using NA-MUG media.
\20\ USEPA. 2002. Method 1103.1: Escherichia coli (E. coli) In Water By Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC).
U.S. Environmental Protection Agency, Office of Water, Washington D.C. EPA-821-R-02-020.
\21\ USEPA. 2002. Method 1603: Escherichia coli (E. coli) In Water By Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (
modified mTEC). U.S. Environmental Protection Agency, Office of Water, Washington D.C. EPA-821-R-02-023.
\22\ Preparation and use of MI agar with a standard membrane filter procedure is set forth in the article, Brenner et al. 1993. ``New Medium for the
Simultaneous Detection of Total Coliform and Escherichia coli in Water.'' Appl. Environ. Microbiol. 59:3534-3544 and in USEPA. 2002. Method 1604:
Total Coliforms and Escherichia coli (E. coli) in Water by Membrane Filtration by Using a Simultaneous Detection Technique (MI Medium). U.S.
Environmental Protection Agency, Office of Water, Washington DC. EPA 821-R-02-024.
\23\ A description of the Enterolert ® test may be obtained from IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092.
\24\ USEPA. 2002. Method 1106.1: Enterococci In Water By Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA). U.S. Environmental
Protection Agency, Office of Water, Washington DC. EPA-821-R-02-021.
\25\ USEPA. 2002. Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-ß-D-Glucoside Agar (mEI). U.S.
Environmental Protection Agency, Office of Water, Washington, DC. EPA-821-R-02-022.
\26\ Method 1622 uses filtration, concentration, immunomagnetic separation of oocysts from captured material, immunofluorescence assay to determine
concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the detection of Cryptosporidium.
USEPA. 2001. Method 1622: Cryptosporidium in Water by Filtration/IMS/FA. U.S. Environmental Protection Agency, Office of Water, Washington DC. EPA-821-
R-01-026.
\27\ Method 1623 uses filtration, concentration, immunomagnetic separation of oocysts and cysts from captured material, immunofluorescence assay to
determine concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the simultaneous detection
of Cryptosporidium and Giardia oocysts and cysts. USEPA. 2001. Method 1623. Cryptosporidium and Giardia in Water by Filtration/IMS/FA. U.S.
Environmental Protection Agency, Office of Water, Washington DC. EPA-821-R-01-025.
\28\ Recommended for enumeration of target organism in ambient water only.
\29\ USEPA. October 2002. Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms. Fifth Edition.
U.S. Environmental Protection Agency, Office of Water, Washington DC. EPA/821/R-02/012.
\30\ USEPA. October 2002. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms. Fourth
Edition. U.S. Environmental Protection Agency, Office of Water, Washington DC. EPA/821/R-02/013.
\31\ USEPA. October 2002. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms.
Third Edition. U.S. Environmental Protection Agency, Office of Water, Washington DC. EPA/821/R-02/014.




Table IB_List of Approved Inorganic Test Procedures
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Reference (method number or page)
Parameter, units and method --------------------------------------------------------------------------------------------------------------------------------------------------------------
EPA 1,35 Standard Methods [Edition(s)] ASTM USGS \2\ Other
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1. Acidity, as CaCO3, mg/L:
Electrometric endpoint or 305.1......................... 2310 B(4a) [18th, 19th, 20th]. D1067-92...................... I-1020-85
phenolphthalein endpoint.
I-2030-85
2. Alkalinity, as CaCO3, mg/L:
Electrometric of Colorimetric 310.1......................... 2320 B [18th, 19th, 20th]..... D1067-92...................... I-1030-85..................... 973.43 \3\
titration to pH 4.5, manual .............................. .............................. .............................. ..............................
or automatic. 310.2......................... .............................. I-2030-85.....................
3. Aluminium_Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \36\.... 202.1......................... 3111 D [18th, 19th]........... .............................. I-3051-85
AA furnace................... 202.2......................... 3113 B [18th, 19th]...........
Inductively Coupled Plasma/ 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\ .............................
Atomic Emission Spectrometry
(ICP/AES) \36\.
Direct Current Plasma (DCP) .............................. .............................. D4190-94...................... .............................. Note 34.
\36\.
Colorimetric (Eriochrome .............................. 3500-Al B [20th] and 3500-Al D
cyanine R). [18th, 19th].
4. Ammonia (as N), mg/L:
Manual, distillation (at pH 350.2......................... 4500-NH3 B [18th, 19th, 20th]. .............................. .............................. 973.49 \3\
9.5) \6\ followed by.
Nesslerization............... 350.2......................... 4500-NH3 C [18th]............. D1426-98(A)................... I-3520-85..................... 973.49 \3\
Titration.................... 350.2......................... 4500-NH3 C [19th, 20th] and
4500-NH3 E [18th].
Electrode.................... 350.3......................... 4500-NH3 D or E [19th, 20th] D1426-98(B)...................
and 4500-NH3 F or G [18th].
Automated phenate, or........ 350.1......................... 4500-NH3 G [19th, 20th] and .............................. I-4523-85 .............................
4500-NH3 H [18th].
Automated electrode.......... .............................. .............................. .............................. .............................. Note 7.
5. Antimony-Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \36\.... 204.1......................... 3111 B [18th, 19th]
AA furnace................... 204.2......................... 3113 B [18th, 19th]...........
ICP/AES \36\................. 200.7 \5\..................... 3120 B [18th, 19th, 20th].....
6. Arsenic-Total \4\ mg/L:
Digestion \4\ followed by.... 206.5.........................
AA gaseous hydride........... 206.3......................... 3114 B 4.d [18th, 19th]....... D2972-97(B) I-3062-85
AA furnace................... 206.2......................... 3113 B [18th, 19th]........... D2972-97(C) I-4063-98 \49\ .............................
ICP/AES \36\ or.............. 200.7 \5\..................... 3120 B [18th, 19th, 20th].....
Colorimetric (SDDC).......... 206.4......................... 3500-As B [20th] and 3500-As C D2972-97(A) I-3060-85
[18th, 19th].
7. Barium-Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \14\.... 208.1......................... 3111 D [18th, 19th]........... .............................. I-3084-85
AA furnace................... 208.2......................... 3113 B [18th, 19th]........... D4382-95
ICP/AES \14\................. 200.7 \5\..................... 3120 B [18th, 19th, 20th].....
DCP \14\..................... .............................. .............................. .............................. .............................. Note 34.
8. Beryllium-Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration......... 210.1......................... 3111 D [18th, 19th]........... D3645-93(88)(A)............... I-3095-85
AA furnace................... 210.2......................... 3113 B [18th, 19th]........... D3645-93(88)(B)
ICP/AES...................... 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\ .............................
DCP, or...................... .............................. .............................. D4190-94...................... .............................. Note 34.
Colorimetric (aluminon....... .............................. 3500-Be D [18th, 19th]........
9. Biochemical oxygen demand
(BOD5), mg/L:
Dissolved Oxygen Depletion... 405.1......................... 5210 B [18th, 19th, 20th]..... .............................. I-1578-78 \8\................. 973.44, \3\ p. 17 \9\
10. Boron \37\-Total, mg/L:
Colorimetric (curcumin)...... 212.3......................... 4500-B B [18th, 19th, 20th]... .............................. I-3112-85
ICP/AES, or.................. 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\ .............................
DCP.......................... .............................. .............................. D4190-94...................... .............................. Note 34.
11. Bromide, mg/L:
Titrimetric.................. 320.1......................... .............................. D1246-95(C)................... I-1125-85..................... p. S44 \10\
12. Cadmium_Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \36\.... 213.1......................... 3111 B or C [18th, 19th]...... D3557-95 (A or B)............. I-3135-85 or I-3136-85........ 974.27, \3\ p. 37 \9\
AA furnace................... 213.2......................... 3113 B [18th, 19th]........... D3557-95(D)................... I-4138-89 \51\
ICP/AES \36\................. 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-1472-85 or I-4471-97 \50\
DCP \36\..................... .............................. .............................. D4190-94...................... .............................. Note 34.
Voltametry \11\, or.......... .............................. .............................. D3557-95(C)...................
Colorimetric (Dithizone)..... .............................. 3500-Cd D [18th, 19th]........
13. Calcium_Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration......... 215.1......................... 3111 B [18th, 19th]........... D511-93(B).................... I-3152-85
ICP/AES...................... 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\
DCP, or...................... .............................. .............................. .............................. .............................. Note 34.
Titrimetric (EDTA)........... 215.2......................... 3500-Ca B [20th] and 3500-Ca D D511-93(A)....................
[18th, 19th].
14. Carbonaceous biochemical
oxygen demand (CBOD 3), mg/L
\12\:
Dissolved Oxygen Depletion .............................. 5210 B [18th, 19th, 20th].....
with nitrification inhibitor.
15. Chemical oxygen demand (COD), 410.1......................... 5220 C [18th, 19th, 20th]..... D1252-95(A)................... I-3560-85..................... 973.46, \3\ p. 17 \9\
mg/L; Titrimetric
or........................... 410.2......................... .............................. .............................. I-3562-85
410.3.........................
Spectrophotometric, manual or 410.4......................... 5220 D [18th, 19th, 20th]..... D1252-95(B)................... I-3561-85..................... Notes 13, 14.
automatic.
16. Chloride, mg/L:
Titrimetric (silver nitrate) .............................. 4500-Cl-B [18th, 19th, 20th].. D512-89(B).................... I-1183-85
or.
(Mercuric nitrate)........... 325.3......................... 4500-Cl-C [18th, 19th, 20th].. D512-89(A).................... I-1184-85..................... 973.51 \3\
Colorimetric, manual or...... .............................. .............................. .............................. I-1187-85
Automated (Ferricyanide)..... 325.1 or 325.2................ 4500-Cl-E [18th, 19th, 20th].. .............................. I-2187-85
17. Chlorine_Total residual, mg/
L; Titrimetric:
Amperometric direct.......... 330.1......................... 4500-Cl D [18th, 19th, 20th].. D1253-86(92)..................
Iodometric direct............ 330.3......................... 4500-Cl B [18th, 19th, 20th]..
Back titration ether end- 330.2......................... 4500-Cl C [18th, 19th, 20th]..
point \15\ or.
DPD-FAS...................... 330.4......................... 4500-Cl F [18th, 19th, 20th]..
Spectrophotometric, DPD...... 330.5......................... 4500-Cl G [18th, 19th, 20th]..
Or Electrode................. .............................. .............................. .............................. .............................. Note 16.
18. Chromium VI dissolved, mg/L;
0.45 micron filtration followed
by:
AA chelation-extraction or... 218.4......................... 3111 C [18th, 19th]........... .............................. I-1232-85
Colorimetric .............................. 3500-Cr B [20th] and 3500-Cr D D1687-92(A)................... I-1230-85
(Diphenylcarbazide). [18th, 19th].
19. Chromium-Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \36\.... 218.1......................... 3111 B [18th, 19th]........... D1687-92(B)................... I-3236-85..................... 974.27 \3\
AA chelation-extraction...... 218.3......................... 3111 C [18th, 19th]...........
AA furnace................... 218.2......................... 3113 B [18th, 19th]........... D1687-92(C)................... I-3233-93 \46\................
ICP/AES \36\................. 200.7 \5\..................... 3120 B [18th, 19th, 20th].....
DCP \36\ or.................. .............................. .............................. D4190-94...................... .............................. Note 34.
Colorimetric .............................. 3500-Cr B [20th] and 3500-Cr D
(Diphenylcarbazide). [18th, 19th].
20. Cobalt_Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration......... 219.1......................... 3111 B or C [18th, 19th]...... D3558-94(A or B).............. I-3239-85..................... p. 37 \9\
AA furnace................... 219.2......................... 3113 B [18th, 19th]........... D3558-94(C)................... I-4243-89 \51\................
ICP/AES...................... 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\................
DCP.......................... .............................. .............................. D4190-94...................... .............................. Note 34.
21. Color platinum cobalt units
or dominant wavelength, hue,
luminance purity:
Colorimetric (ADMI), or......
(Platinum cobalt), or........ 110.1......................... 2120 E [18th, 19th, 20th]..... .............................. .............................. Note 18.
Spectrophotometric........... 110.2......................... 2120 B [18th, 19th, 20th]..... .............................. I-1250-85
110.3......................... 2120 C [18th, 19th, 20th].....
22. Copper_Total, 4 mg/L;
Digestion 4 followed by:
AA direct aspiration 36...... 220.1......................... 3111 B or C [18th, 19th]...... D1688-95(A or B).............. I-3270-85 or I-3271-85........ 974.27 3 p. 37 9
AA furnace................... 220.2......................... 3113 B [18th, 19th]........... D1688-95(C)................... I-4274-89 51
ICP/AES 36................... 200.7 5....................... 3120 B [18th, 19th, 20th]..... .............................. I_4471_97 50
DCP 36 or.................... .............................. .............................. D4190-94...................... .............................. Note 34.
Colorimetric (Neocuproine) or .............................. 3500-Cu B [20th] and 3500-Cu D
[18th, 19th].
(Bicinchoninate)............. .............................. 3500-Cu C [20th] and 3500-As B .............................. .............................. Note 19.
[18th, 19th].
23. Cyanide_Total, mg/L:
Manual distillation with .............................. 4500-CN C [18th, 19th, 20th].. D2036-98(A)
MgCl2 followed by..
Titrimetric, or.............. .............................. 4500-CN D [18th, 19th, 20th].. .............................. .............................. p. 22 9
Spectrophotometric, manual or 335.2 31...................... 4500-CN E [18th, 19th, 20th].. D2036-98(A)................... I-3300-85
Automated 20................. 335.3 31...................... .............................. .............................. I-4302-85
24. Available Cyanide, mg/L:
Manual distillation with 335.1......................... 4500-CN G [18th, 19th, 20th].. D2036-98(B)
MgCl2 followed by
titrimetric or
Spectrophotometric.
Flow injection and ligand .............................. .............................. .............................. .............................. OIA-1677 44
exchange, followed by
amperometry.
25. Fluoride_Total, mg/L:
Manual distillation 6 .............................. 4500-F B [18th, 19th, 20th]...
followed by.
Electrode, manual or......... 340.2......................... 4500-F C [18th, 19th, 20th]... D1179-93(B)
Automated.................... .............................. .............................. .............................. I-4327-85
Colorimetric (SPADNS)........ 340.1......................... 4500-F D [18th, 19th, 20th]... D1179-93(A)
Or Automated complexone...... 340.3......................... 4500-F E [18th, 19th, 20th]...
26. Gold_Total, 4 mg/L; Digestion
4 followed by:
AA direct aspiration......... 231.1......................... 3111 B [18th, 19th]...........
AA furnace, or............... 231.2
DCP.......................... .............................. .............................. .............................. .............................. Note 34.
27. Hardness_Total, as CaCO3, mg/
L:
Automated colorimetric,...... 130.1
Titrimetric (EDTA), or Ca 130.2......................... 2340 B or C [18th, 19th, 20th] D1126-86(92).................. I-1338-85..................... 973.52B 3
plus Mg as their carbonates,
by inductively coupled
plasma or AA direct
aspiration (See Parameters
13 and 33).
28. Hydrogen ion (pH), pH units:
Electrometric measurement, or 150.1......................... 4500-H+ B [18th, 19th, 20th].. D1293-84 (90)(A or B)......... I-1586-85..................... 973.41 3
Automated electrode.......... .............................. .............................. .............................. I-2587-85..................... Note 21.
29. Iridium_Total, 4 mg/L;
Digestion 4 followed by:
AA direct aspiration or...... 235.1......................... 3111 B [18th, 19th]...........
AA furnace................... 235.2
30. Iron_Total, 4 mg/L; Digestion
4 followed by:
AA direct aspiration 36...... 236.1......................... 3111 B or C [18th, 19th]...... D1068-96(A or B).............. I-3381-85..................... 974.27 3
AA furnace................... 236.2......................... 3113 B [18th, 19th]........... D1068-96(C)
ICP/AES 36................... 200.7 5....................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 50
DCP 36 or.................... .............................. .............................. D4190-94...................... .............................. Note 34.
Colorimetric (Phenanthroline) .............................. 3500-Fe B [20th] and 3500-Fe D D1068-96(D)................... .............................. Note 22.
[18th, 19th].
31. Kjeldahl Nitrogen_Total, (as
N), mg/L:
Digestion and distillation 351.3......................... 4500-Norg B or C and 4500-NH3 D3590-89(A)
followed by. B [18th, 19th, 20th].
Titration.................... 351.3......................... .............................. D3590-89(A)................... .............................. 973.48 \3\
Nesslerization............... 351.3......................... 4500-NH3 C [18th]............. D3590-89(A)...................
Electrode.................... 351.3......................... 4500-NH3 C [19th, 20th] and
4500-NH3 E [18th].
Automated phenate colorimetric... 351.1......................... .............................. .............................. I-4551-78 \8\
Semi-automated block digestor 351.2......................... .............................. D3590-89(B)................... I-4515-91 \45\................
colorimetric.
Manual or block digestor 351.4......................... .............................. D3590-89(A)
potentiometric.
Block digester, followed by Auto .............................. .............................. .............................. .............................. Note 39.
distillation and Titration, or.
Nesslerization, or............... .............................. .............................. .............................. .............................. Note 40.
Flow injection gas diffusion..... .............................. .............................. .............................. .............................. Note 41.
32. Lead_Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \36\.... 239.1......................... 3111 B or C [18th, 19th]...... D3559-96(A or B).............. I-3399-85..................... 974.27 \3\
AA furnace................... 239.2......................... 3113 B [18th, 19th]........... D3559-96(D)................... I-4403-89 \51\
ICP/AES \36\................. 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\
DCP 36....................... .............................. .............................. D4190-94...................... .............................. Note 34.
Voltametry \11\ or........... D3559-96(C)...................
Colorimetric (Dithizone)..... 3500-Pb B [ 20th] and 3500-Pb
D [18th, 19th].
33. Magnesium_Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration......... 242.1......................... 3111 B [18th, 19th]........... D511-93(B).................... I-3447-85..................... 974.27 \3\
ICP/AES...................... 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\
DCP or....................... .............................. .............................. .............................. .............................. Note 34.
Gravimetric.................. .............................. 3500-Mg D [18th, 19th]........
34. Manganese-Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \36\.... 243.1......................... 3111 B [18th, 19th]........... D858-95(A or B)............... I-3454-85..................... 974.27 \3\
AA furnace................... 243.2......................... 3113 B [18th, 19th]........... D858-95(C)
ICP/AES \36\................. 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\
DCP \36\, or................. .............................. .............................. D4190-94...................... .............................. Note 34
Colorimetric (Persulfate), or .............................. 3500-Mn B [20th] and 3500-Mn D .............................. .............................. 920.203 \3\
[18th, 19th].
(Periodate).................. .............................. .............................. .............................. .............................. Note 23.
35. Mercury_Total, \4\ mg/L:
Cold vapor, manual or........ 245.1......................... 3112 B [18th, 19th]........... D3223-91...................... I-3462-85..................... 977.22 \3\
Automated.................... 245.2
Oxidation, purge and trap, 1631E \43\
and cold vapor atomic
fluorescence spectrometry
(ng/L).
36. Molybdenum_Total \4\, mg/L;
Digestion \4\ followed by:
AA direct aspiration......... 246.1......................... 3111 D [18th, 19th]........... .............................. I-3490-85
AA furnace................... 246.2......................... 3113 B [18th, 19th]........... .............................. I-3492-96 \47\
ICP/AES...................... 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\
DCP.......................... .............................. .............................. .............................. .............................. Note 34.
37. Nickel_Total, \4\ mg/L;
Digestion \4\ followed by:
AA direct aspiration \36\.... 249.1......................... 3111 B or C [18th, 19th]...... D1886-90(A or B).............. I-3499-85.....................
AA furnace................... 249.2......................... 3113 B [18th, 19th]........... D1886-90(C)................... I-4503-89 \51\................
ICP/AES \36\................. 200.7 \5\..................... 3120 B [18th, 19th, 20th]..... .............................. I-4471-97 \50\................
DCP \36\, or................. .............................. .............................. D4190-94...................... .............................. Note 34.
Colorimetric (heptoxime)..... .............................. 3500-Ni D [17th]..............
38. Nitrate (as N), mg/L:
Colorimetric (Brucine 352.1......................... .............................. .............................. .............................. 973.50, \3\ 419D, \17\ p. 28
sulfate), or Nitrate-nitrite \9\
N minus Nitrite N (See
parameters 39 and 40).
39. Nitrate-nitrite (as N),
mg/L:
Cadmium reduction, Manual or. 353.3......................... 4500-NO3-E [18th, 19th, 20th]. D3867-99(B)...................
Automated, or................ 353.2......................... 4500-NO3-F [18th, 19th, 20th]. D3867-99(A)................... I-4545-85.....................
Automated hydrazine.......... 353.1......................... 4500-NO3-H [18th, 19th, 20th].
40. Nitrite (as N), mg/L;
Spectrophotometric:
Manual or.................... 354.1......................... 4500-NO2-B [18th, 19th, 20th]. .............................. .............................. Note 25.
Automated (Diazotization).... .............................. .............................. .............................. I-4540-85.....................
41. Oil and grease_Total
recoverable, mg/L:
Gravimetric (extraction)..... 413.1......................... 5520B [18th, 19th, 20th] \38\.
Oil and grease and non-polar 1664A \42\.................... 5520B [18th, 19th, 20th] \38\.
material, mg/L: Hexane
extractable material (HEM):
n-Hexane extraction and
gravimetry.
Silica gel treated HEM (SGT- 1664A \42\....................
HEM): Silica gel treatment
and gravimetry.
42. Organic carbon_Total (TOC),
mg/L:
Combustion or oxidation...... 415.1......................... 5310 B, C, or D [18th, 19th, D2579-93 (A or B)............. .............................. 973.47, \3\ p. 14 \24\
20th].
43. Organic nitrogen (as N), mg/
L:
Total Kjeldahl N (Parameter
31) minus ammonia N
(Parameter 4).
44. Orthophosphate (as P), mg/L;
Ascorbic acid method:
Automated, or................ 365.1......................... 4500-P F [18th, 19th, 20th]... .............................. I-4601-85..................... 973.56 \3\
Manual single reagent........ 365.2......................... 4500-P E [18th, 19th, 20th]... D515-88(A).................... .............................. 973.55 \3\
Manual two reagent........... 365.3.........................
45. Osmium_Total \4\, mg/L;
Digestion \4\ followed by:
AA direct aspiration, or..... 252.1......................... 3111 D [18th, 19th]........... (continued)